اندازه گیری سیالوپروتئین سرم و بررسی ارتباط آن با تغییرات ساختمانی مینا و عاج دندان

Sialoprotein is a phosphorylated skeletal glycoprotein. The function of sialoprotein is still not fully understood. It is possible that this component participate in cell differentiation and the conversion of unmineralized matrix into mineralized structures. The purpose of the present study was to determine the serum sialoprotein concentration in-patient and normal subjects to investigate the relationship between serum sialoprotein and dentin, enamel structural differentiation. The study group consisted of 50 patients, 32 males and 18 females, aged 17-54 (with an average of 32.5 years). The control group consisted of 50 normal volunteers, 26 males and 24 females, age 22-51 (with an average of 39.60 years).The laboratory data of this group were used as a reference. Human blood was obtained from control and patient group. The blood sample was centrifuged at 3000* g for 20 min, the supernatant was discarded.Then each sample was centrifuged for 20 min at 10000 g applied to a sepharose column and eluted with 4 moll Guanidine - HCL at 50 moll tris, pH 7.4 at a flow rate of 0.5 ml/min. The fractions obtained by chromatograply was monitored by electrophoresis. Our results showed that sialoprotein control increased in patients serum (18.45+/-3.2I micro gr/L) compared to normal subjects (11.37+/-2.45 micro gr/L).